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Fig. 1. Gene profiles of genome-wide CRISPR screen. A. Cells were treated with different doses of AAI for 48 h. After exposure, cell viability was measured by the MTS assay. B. Schematic diagram for genome-wide CRISPR screen. C. Volcano plot for candidate genes. D. Core analysis of candidate genes. E. Tox List for candidate genes. F. Disease and function plot for candidate genes. Data are presented as the mean ± SEM.

Journal: Environment international

Article Title: Genome-wide CRISPR-based screen identifies E2F transcription factor 1 as a regulator and therapeutic target of aristolochic acid-induced nephrotoxicity.

doi: 10.1016/j.envint.2024.109234

Figure Lengend Snippet: Fig. 1. Gene profiles of genome-wide CRISPR screen. A. Cells were treated with different doses of AAI for 48 h. After exposure, cell viability was measured by the MTS assay. B. Schematic diagram for genome-wide CRISPR screen. C. Volcano plot for candidate genes. D. Core analysis of candidate genes. E. Tox List for candidate genes. F. Disease and function plot for candidate genes. Data are presented as the mean ± SEM.

Article Snippet: The GeCKO v2 genome-scale CRISPR knockout library was obtained from Addgene (Plasmid #52961) and contains 65,383 single-guide RNAs (sgRNAs) targeting 19,050 genes.

Techniques: Genome Wide, CRISPR, MTS Assay